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"Modelling glaucoma with pluripotent stem cells" by Katherine Gill
29.03.2016 |
| Dato | tor 31 mar |
| Tid | 11:00 — 12:00 |
| Sted | Biokemi 4 (building 1170, 3rd floor, room 340), Ole Worms Allé 3, Aarhus University |
Katherine Gill
Glaucoma is a heterogeneous group of optic neuropathies that culminate in retinal ganglion cell (RGCs) apoptosis leading to vision loss. Glaucoma is an insidious disease with a complex aetiology. A major barrier to studying glaucoma is restricted access to the RGCs within the retina pre-mortem coupled with lack of appropriate animal models. Induced pluripotent stem cell (iPSC) technology offers the ability to interrogate disease pathophysiology in vitro. In this project, patient-derived iPSCs were generated from a cohort of individuals affected or unaffected with primary open angle glaucoma (POAG) carrying homozygous risk variants associated with POAG. Using an optimized differentiation protocol, RGCs were generated from these iPSC lines. These iPSC-derived RGCs were used to investigate disease-specific phenotypes in vitro for investigating the pathophysiology of glaucomatous loss of RGCs. Further, analysis of these iPSC-derived RGCs by next generation sequencing (RNAseq) was performed to investigate the molecular basis of POAG by identification of a series of genes and signaling pathways. Together, the generation of iPSC-derived RGCs will provide a major resource for understanding the molecular pathogenesis of POAG.
Host: Group Leader Mark Denham, DANDRITE, Aarhus University